PARP4 Polyclonal antibody

• 

  WB analysis using 19775-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 19775-1-AP (PARP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19775-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19775-1-AP (PARP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 19775-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19775-1-AP (PARP4 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 19775-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed 48孔 HepG2 cells using PARP4 antibody (19775-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

PARP4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 19775-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PARP 4, p193, EC:2.4.2.-, ARTD4, ADPRTL1

---

Tested Applications

• Positive WB detected in: A549 cells, HeLa cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: 48孔 HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19775-1-AP targets PARP4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: poly (ADP-ribose) polymerase family, member 4
• Calculated Molecular Weight: 193 kDa
• Observed Molecular Weight: 193 kDa
• GenBank Accession Number: NM_006437
• Gene Symbol: PARP4
• Gene ID (NCBI): 143
• RRID: AB_3085585
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UKK3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PARP4, also named as ADPRTL1, KIAA0177, PARPL, VPARP and PH5P, catalyzes the reaction: NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.