ChromoTek PARP1-Chromobody® plasmid (TagRFP)

DNA plasmid encoding for anti-PARP1 VHH (anti-PARP1 Nanobody) fused to TagRFP.

Specificity

PARP1

Applications

IF, Live cell imaging

Vector type

Mammalian expression vector

Reporter

TagRFP

Publications

7

Cat no : xcr

Synonyms

Chromobody, PARP1 Chromobody

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Validation Data Gallery

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  Time series of imaging DNA damage in HeLa cells after microirradiation: The PARP1-Chromobody allows real-time monitoring of PARP1 recruitment to the location of DNA damage after microirradiation (yellow triangle) in living cells.

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Product Information

DNA plasmid encoding for anti-PARP1 VHH (anti-PARP1 Nanobody) fused to TagRFP.

• Description: The PARP1-Chromobody visualizes endogenous PARP1 in real time in live cells.

  • Easily transfect your cells

  • Use PARP1-Chromobody® as marker of DNA damage after microirradiation

  • PARP1-Chromobody® does not affect cell viability

• Applications: Live cell imaging
• Specificity: Poly(ADP-ribose) polymerase 1 (PARP1), tested in human cells.; Does not bind to PARP2, PARP3, andPARP 9
• Reporter: TagRFP
• Vector type: Mammalian expression vector
• Promoter: Constitutive CMV IE
• Codon usage: Mammalian
• Selection: Kan/Neo
• Sequence: With the PARP1-Chromobody plasmid you receive the sequence information of the alpaca antibody to PARP1 fused to TagGFP2 or TagRFP, as well as the full vector sequence.
• Transfection: Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Fisher Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
• Microscopy techniques: Wide-field epifluorescence microscopy; confocal microscopy
• Storage Condition: Shipped at ambient temperature. Store at -20 °C

Publications

Application	Title
	Nat Commun; SPINDOC binds PARP1 to facilitate PARylation.; Authors - Fen Yang
	Proc Natl Acad Sci U S A; Rapid recruitment of p53 to DNA damage sites directs DNA repair choice and integrity.; Authors - Yu-Hsiu Wang
IF	J Cell Sci; Local inhibition of rRNA transcription without nucleolar segregation after targeted ion irradiation of the nucleolus.; Authors - Christian Siebenwirth
IF	J Biophotonics; Chromatin nanoscale compaction in live cells visualized by acceptor-to-donor ratio corrected Förster resonance energy transfer between DNA dyes.; Authors - Simone Pelicci
	Nucleic Acids Res; The loss of DNA polymerase epsilon accessory subunits POLE3-POLE4 leads to BRCA1-independent PARP inhibitor sensitivity; Authors - Hasan Mamar
Cell treatment	Sci Rep; Segmented fluorescence correlation spectroscopy (FCS) on a commercial laser scanning microscope; Authors - Elisa Longo