PARN Polyclonal antibody

PARN Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 13799-1-AP

Synonyms

DAN, Deadenylating nuclease, Deadenylation nuclease, PARN

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Validation Data Gallery

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  WB analysis using 13799-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13799-1-AP (PARN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human cerebellum using 13799-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 13799-1-AP (PARN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human endometrial cancer using 13799-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 13799-1-AP (PARN antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human endometrial cancer using 13799-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 13799-1-AP (PARN antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells, human cerebellum tissue, Jurkat cell, MDA-MB-453s cells
• Positive IHC detected in: human endometrial cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

13799-1-AP targets PARN in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PARN fusion protein Ag4774
• Full Name: poly(A)-specific ribonuclease (deadenylation nuclease)
• Calculated Molecular Weight: 639 aa, 73 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC050029
• Gene Symbol: PARN
• Gene ID (NCBI): 5073
• RRID: AB_2160430
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IHC	Hum Mol Genet; CGG repeats in RNA modulate expression of TDP-43 in mouse and fly models of fragile X tremor ataxia syndrome.; Authors - Jocelyn N Galloway
human	IF	Neurobiol Dis; Distinct Poly(A) nucleases have differential impact on sut-2 dependent tauopathy phenotypes.; Authors - Rebecca L Kow
mouse	WB	Cell Rep; YTHDF2/3 Are Required for Somatic Reprogramming through Different RNA Deadenylation Pathways.; Authors - Jiadong Liu
human	WB	Nucleic Acids Res; Molecular mechanism of human ISG20L2 for the ITS1 cleavage in the processing of 18S precursor ribosomal RNA; Authors - Yinliang Ma