PARK7/DJ-1 Recombinant antibody, PBS Only

PARK7/DJ-1 Uni-rAb^TM Recombinant Antibody for ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

ELISA, WB

Conjugate

Unconjugated

CloneNo.

230124B3

Cat no : 82913-3-PBS

Synonyms

PARK7, PARK7,DJ-1, Maillard deglycase, EC:3.1.2.-, EC:3.5.1.-, EC:3.5.1.124, DJ-1

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Validation Data Gallery

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  WB analysis using 82913-3-RR (same clone as 82913-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82913-3-RR (PARK7/DJ-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82913-3-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 82913-3-RR (same clone as 82913-3-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug PARK7/DJ-1 Recombinant antibody (82913-3-RR, Clone:230124B3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82913-3-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82913-3-RR

  Biolayer interferometry (BLl) kinetic assays of 82913-3-RR against Human PARK7/DJ-1 were performed. The affinity constant is 1.02 nM.

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Product Information

The immunogen of 82913-3-PBS is PARK7/DJ-1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PARK7/DJ-1 fusion protein Ag2287
• Full Name: Parkinson disease (autosomal recessive, early onset) 7
• Calculated Molecular Weight: 189 aa, 20 kDa
• GenBank Accession Number: BC008188
• Gene Symbol: PARK7
• Gene ID (NCBI): 11315
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.