PARG Polyclonal antibody

PARG Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (2)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 27808-1-AP

Synonyms

PARG, PARG99

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Validation Data Gallery

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  WB analysis of K-562 using 27808-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 27808-1-AP (PARG antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 27808-1-AP

  IP result of anti-PARG (IP:27808-1-AP, 4ug; Detection:27808-1-AP 1:300) with mouse testis tissue lysate 3920 ug.

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  IHC staining of human breast cancer using 27808-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27808-1-AP (PARG antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27808-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27808-1-AP (PARG antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: K-562 cells
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

27808-1-AP targets PARG in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Cited Reactivity: human, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PARG fusion protein Ag27137
• Full Name: poly (ADP-ribose) glycohydrolase
• Calculated Molecular Weight: 111 kDa
• Observed Molecular Weight: 100-111 kDa
• GenBank Accession Number: BC052966
• Gene Symbol: PARG
• Gene ID (NCBI): 8505
• RRID: AB_2880979
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Theranostics; Hepatoma cell-intrinsic TLR9 activation induces immune escape through PD-L1 upregulation in hepatocellular carcinoma.; Authors - Binghai Zhou
zebrafish	WB	Int J Mol Sci; Synthesis and Degradation of Poly(ADP-ribose) in Zebrafish Brain Exposed to Aluminum; Authors - Anna Rita Bianchi
human	WB,IF	Viruses; Role of PARP-1 in Human Cytomegalovirus Infection and Functional Partners Encoded by This Virus; Authors - Wenchang Zhang