PARD3 Monoclonal antibody, PBS Only

PARD3 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D2B2

Cat no : 66968-1-PBS

Synonyms

ASIP, Baz, Bazooka, CTCL tumor antigen se2 5, FLJ21015, PAR 3, PAR3, PAR3 alpha, PAR3A, PAR3alpha, PARD 3, PARD3, PARD3A, SE2 5L16, SE2 5LT1, SE2 5T2

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Validation Data Gallery

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  WB analysis of MCF-7 using 66968-1-Ig (same clone as 66968-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66968-1-Ig (PARD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66968-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66968-1-Ig (same clone as 66968-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66968-1-Ig (PARD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66968-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66968-1-Ig (same clone as 66968-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66968-1-Ig (PARD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66968-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66968-1-Ig (same clone as 66968-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66968-1-Ig (PARD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66968-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 66968-1-Ig (same clone as 66968-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66968-1-Ig (PARD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66968-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 66968-1-Ig (same clone as 66968-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PARD3 antibody (66968-1-Ig, Clone: 1D2B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66968-1-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 66968-1-Ig (same clone as 66968-1-PBS)

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human PARD3 (66968-1-Ig, Clone:1D2B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66968-1-PBS in a different storage buffer formulation.

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Product Information

66968-1-PBS targets PARD3 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PARD3 fusion protein Ag7656
• Full Name: par-3 partitioning defective 3 homolog (C. elegans)
• Calculated Molecular Weight: 151 kDa
• Observed Molecular Weight: 170 kDa
• GenBank Accession Number: BC011711
• Gene Symbol: PARD3
• Gene ID (NCBI): 56288
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PARD3 (also known as ASIP, Par3, or Bazooka) is one of PARD proteins which are essential for asymmetric cell division and polarized growth. PARD3 is involved in the establishment of cell polarity and in the asymmetric cytokinesis. It plays a role in tight junctions at epithelial cell-cell contacts.