PANK1/2/3 Polyclonal antibody

PANK1/2/3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12466-1-AP

Synonyms

hPanK3, PANK1/2/3, PANK3, pantothenate kinase 3, Pantothenic acid kinase 3

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Validation Data Gallery

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  WB analysis of HepG2 using 12466-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12466-1-AP (PANK1/2/3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human hepatocirrhosis using 12466-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 12466-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 12466-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12466-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12466-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 12466-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 12466-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 12466-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IHC detected in: human hepatocirrhosis tissue, human breast cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12466-1-AP targets PANK1/2/3 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PANK1/2/3 fusion protein Ag3140
• Full Name: pantothenate kinase 3
• Calculated Molecular Weight: 370 aa, 41 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC013705
• Gene Symbol: PANK3
• Gene ID (NCBI): 79646
• RRID: AB_10644117
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
sheep	WB	Int J Mol Sci; MicroRNA-200c Affects Milk Fat Synthesis by Targeting PANK3 in Ovine Mammary Epithelial Cells; Authors - Zhiyun Hao