Tested Applications
Positive WB detected in | HeLa cells, Jurkat cells, NIH/3T3 cells |
Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
25655-1-AP targets PAN3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PAN3 fusion protein Ag22559 Predict reactive species |
Full Name | PAN3 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) |
Calculated Molecular Weight | 887 aa, 96 kDa |
Observed Molecular Weight | 65-68 kDa |
GenBank Accession Number | BC128180 |
Gene Symbol | PAN3 |
Gene ID (NCBI) | 255967 |
RRID | AB_3669491 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q58A45 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Shortening of the poly(A) tail in mRNA, called deadenylation, is the first rate-limiting step in mRNA turnover. PAN3 is the regulatory subunit of a cytoplasmic poly(A) nuclease complex that links the catalytic PAN2 subunit to the poly(A)-binding protein PABP, which stimulates PAN2-dependent deadenylation activity (PMID: 14583602). PAN3 has four isoforms, isoform1: 95 kDa; isoform2: 64 kDa, isoform3: 89 kDa and isoform4: 72 kDa. This antibody recognizes isoform2.
Protocols
Product Specific Protocols | |
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WB protocol for PAN3 antibody 25655-1-AP | Download protocol |
IHC protocol for PAN3 antibody 25655-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |