Palladin-Specific Polyclonal antibody

Palladin-Specific Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 16179-1-AP

Synonyms

CGI 151, KIAA0992, palladin, PALLD, PNCA1, Sarcoma antigen NY SAR 77, SIH002

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Validation Data Gallery

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  WB analysis of HeLa using 16179-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16179-1-AP (Palladin-Specific antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16179-1-AP

  IP result of anti-Palladin-Specific (IP:16179-1-AP, 4ug; Detection:16179-1-AP 1:200) with HeLa cells lysate 1500ug.

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16179-1-AP targets Palladin-Specific in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: palladin, cytoskeletal associated protein
• Calculated Molecular Weight: 151 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: NM_016081
• Gene Symbol: palladin
• Gene ID (NCBI): 23022
• RRID: AB_10695626
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Palladin is a widely expressed protein found in stress fibers, focal adhesions, growth cones, Z-discs, and other actin-based subcellular structures. It belongs to a small gene family that includes the Z-disc proteins myopalladin and myotilin. It is a cytoskeletal protein required for organization of normal actin cytoskeleton. It functions in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. This antibody, generated against N-terminal peptide of palladin, recognizes 200 kDa, 140 kDa, and 65 kDa isoforms of this protein.