PAK7 Recombinant antibody, PBS Only

PAK7 Recombinant Antibody for WB, IF, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230201A10

Cat no : 82935-1-PBS

Synonyms

KIAA1264, p21 activated kinase 5, p21 activated kinase 7, PAK 5, PAK 7, PAK5, PAK7

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Validation Data Gallery

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  WB analysis using 82935-1-RR (same clone as 82935-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82935-1-RR (PAK7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82935-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82935-1-RR (same clone as 82935-1-PBS)

  WB result of PAK7 antibody (82935-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAK7 transfected HeLa cells. This data was developed using the same antibody clone with 82935-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 82935-1-RR (same clone as 82935-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PAK7 antibody (82935-1-RR, Clone: 230201A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 82935-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82935-1-PBS targets PAK7 in WB, IF, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PAK7 fusion protein Ag29190
• Full Name: p21 protein (Cdc42/Rac)-activated kinase 7
• Calculated Molecular Weight: 719 aa, 81 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC024179
• Gene Symbol: PAK7
• Gene ID (NCBI): 57144
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PAK7(p21-activated kinase 7) is also named as KIAA1264, PAK5 and belongs to the STE Ser/Thr protein kinase family. It plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival.