PAK6 Polyclonal antibody

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  WB analysis using 13539-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13539-1-AP (PAK6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 13539-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 13539-1-AP (PAK6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 13539-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13539-1-AP (PAK6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 13539-1-AP

  IP result of anti-PAK6 (IP:13539-1-AP, 4ug; Detection:13539-1-AP 1:300) with mouse testis tissue lysate 4000 ug.

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  IHC staining of human prostate cancer using 13539-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13539-1-AP (PAK6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 13539-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13539-1-AP (PAK6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 13539-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PAK6 antibody (13539-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

PAK6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13539-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

PAK5, PAK 6, PAK 5, p21-activated kinase 6, p21 activated kinase 6

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Tested Applications

• Positive WB detected in: mouse testis tissue, Raji cells, rat testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Product Information

13539-1-AP targets PAK6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PAK6 fusion protein Ag4467
• Full Name: p21 protein (Cdc42/Rac)-activated kinase 6
• Calculated Molecular Weight: 681 aa, 75 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC035596
• Gene Symbol: PAK6
• Gene ID (NCBI): 56924
• RRID: AB_2158467
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NQU5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAK6(p21-activated kinase 6) is a serine/threonine kinase belonging to the p21-activated kinase (PAK) family. .This protein plays a role in the regulation of gene transcription and may protect cells from apoptosis through phosphorylation of BAD. Northern blot analysis revealed that PAK6 is highly expressed in testis and prostate tissues (PMID:11278661). The immunogen of this antibody is the C-terminal of PAK6. This antibody has weak cross-reaction with other PAK members.

Publications

Species	Application	Title
human	WB,IHC	Oncol Lett; PAK6 promotes cervical cancer progression through activation of the Wnt/β-catenin signaling pathway.; Authors - Qin Yang ; KD Validated
rat	WB	J Pain Res; Proteomics analysis of the amygdala in rats with CFA-induced pain aversion with electro-acupuncture stimulation.; Authors - Yuanyuan Wu
human	WB, IHC	Mol Med Rep; PMID: 26459798; Authors - Chunhui Liu
human	WB	BMC Nephrol; Urinary exosome proteins PAK6 and EGFR as noninvasive diagnostic biomarkers of diabetic nephropathy; Authors - Tao Li