PAK4 Polyclonal antibody

• Featured Product
• KD/KO Validated

PAK4 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

19

Reviews (2)

Cat no : 14685-1-AP

Synonyms

PAK-4, PAK 4, p21-activated kinase 4, p21 activated kinase 4, KIAA1142

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Validation Data Gallery

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  WB analysis of HeLa using 14685-1-AP

  WB result of PAK4 antibody (14685-1-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAK4 transfected HeLa cells.

• 

  WB analysis of COLO 320 using 14685-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14685-1-AP (PAK4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 14685-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14685-1-AP (PAK4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14685-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14685-1-AP (PAK4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 14685-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14685-1-AP (PAK4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skin using 14685-1-AP

  rat skin tissue were subjected to SDS PAGE followed by western blot with 14685-1-AP (PAK4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 14685-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14685-1-AP (PAK4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 14685-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14685-1-AP (PAK4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 14685-1-AP

  IP result of anti-PAK4 (IP:14685-1-AP, 4ug; Detection:14685-1-AP 1:800) with mouse liver tissue lysate 4000ug.

• 

  IF Staining of HeLa using 14685-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PAK4 antibody (14685-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: COLO 320 cells, HeLa cells, PC-3 cells, rat skin tissue
• Positive IP detected in: mouse liver tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 17 publications below
• IHC: See 6 publications below
• IF: See 5 publications below

Product Information

14685-1-AP targets PAK4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PAK4 fusion protein Ag5981
• Full Name: p21 protein (Cdc42/Rac)-activated kinase 4
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC025282
• Gene Symbol: PAK4
• Gene ID (NCBI): 10298
• RRID: AB_2158610
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAK4(p21-activated kinase 4) is also named as KIAA1142. It belongs to the protein kinase superfamily.PAK4 regulates cell morphology, cytoskeletal organization, and cell proliferation and migration. It can also function as an antiapoptotic protein (PMID:14517283). It has 4 isoforms produced by alternative splicing. And it can be autophosphorylated on serine residues when activated by CDC42/p21 (PMID:11278822).

Publications

Species	Application	Title
human	WB,IF	Nat Commun; Proximity proteomics identifies PAK4 as a component of Afadin-Nectin junctions.; Authors - Yohendran Baskaran ; KD Validated
human	WB,IHC	Cancer Res; Targeting PAK4 Inhibits Ras-Mediated Signaling and Multiple Oncogenic Pathways in High-Risk Rhabdomyosarcoma.; Authors - Atreyi Dasgupta
human	IHC,WB	Mol Ther Nucleic Acids; ZEB1-Mediated Transcriptional Upregulation of circWWC3 Promotes Breast Cancer Progression through Activating Ras Signaling Pathway.; Authors - Lingjiao Meng
human	WB	Aging (Albany NY); PAK4 suppresses TNF-induced release of endothelial microparticles in HUVECs cells.; Authors - Shouqin Zhang ; KD Validated
mouse	WB	Commun Biol; RhoGDIα regulates spermatogenesis through Rac1/cofilin/F-actin signaling; Authors - Haixia Zhu
human	WB	Mol Neurobiol; Exosomes Derived from Meningitic Escherichia coli-Infected Brain Microvascular Endothelial Cells Facilitate Astrocyte Activation; Authors - Ruicheng Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Juan (Verified Customer) (05-21-2024)

This primary antibody works pretty good!

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry, Cell culture
• Primary Antibody Dilution: PAK4 Polyclonal antibody
• Cell Tissue Type: human cells

FH

Emily (Verified Customer) (07-18-2022)

Good strong signal by western blot, intend to use for IF soon

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Breast cancer cell