PAK2 Polyclonal antibody

PAK2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 19979-1-AP

Synonyms

p21-activated kinase 2, p21 activated kinase 2, Gamma-PAK, Gamma PAK, EC:2.7.11.1

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Validation Data Gallery

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  WB analysis using 19979-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19979-1-AP (PAK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 19979-1-AP

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 19979-1-AP (PAK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 19979-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19979-1-AP (PAK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 19979-1-AP

  IP result of anti-PAK2 (IP:19979-1-AP, 4ug; Detection:19979-1-AP 1:2000) with K-562 cells lysate 2400 ug.

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  IF Staining of HeLa using 19979-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PAK2 antibody (19979-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, human skeletal muscle tissue, Jurkat cells, HeLa cells, PC-3 cells, Raji cells
• Positive IP detected in: K-562 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19979-1-AP targets PAK2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: p21 protein (Cdc42/Rac)-activated kinase 2
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: NM_002577
• Gene Symbol: PAK2
• Gene ID (NCBI): 5062
• RRID: AB_10644144
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAK2, also named as PAK65, PAKgamma, p58, PAK-2p27, PAK-2p24 and C-t-PAK2, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. PAK2 has several isoforms with the MW of 54-62 kDa and 41 kDa. The 62 kDa PAK2 is cleaved into a 34 kDa C terminal fragment and a 28 kDa N terminal fragment with a time course that parallels apoptotic death in Jurkat cells. (PMID:10200518). Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form. The antibody has no cross reaction to PAK1 and PAK3.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Frédéric (Verified Customer) (02-18-2022)

Je suis satisfait du résultat car j’ai pu détecter par Western-blot une seule bande de forte intensité d’environ 69 kDa sur un lysat de plaquette humaine (voir figure ci-jointe).

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: lysat de plaquette humaine

PAK2 Antibody Western Blot validation (1/1000 dilution) in lysat de plaquette humaine (Cat no:19979-1-AP)