PAIP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PAIP1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 10675-1-AP

Synonyms

Polyadenylate-binding protein-interacting protein 1, Poly(A)-binding protein-interacting protein 1, PAIP-1, PAIP 1, PABP-interacting protein 1

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Validation Data Gallery

• 

  WB analysis of HeLa using 10675-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10675-1-AP (PAIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COS-7 using 10675-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 10675-1-AP (PAIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 10675-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10675-1-AP (PAIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 10675-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10675-1-AP (PAIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10675-1-AP

  IP result of anti-PAIP1 (IP:10675-1-AP, 4ug; Detection:10675-1-AP 1:300) with HeLa cells lysate 3000 ug.

• 

  IF Staining of MCF-7 using 10675-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PAIP1 antibody (10675-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: HeLa cells, BxPC-3 cells, COS-7 cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

10675-1-AP targets PAIP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, monkey samples.

• Tested Reactivity: human, monkey
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PAIP1 fusion protein Ag1070
• Full Name: poly(A) binding protein interacting protein 1
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 60 kDa, 51 kDa, 40 kDa
• GenBank Accession Number: BC005295
• Gene Symbol: PAIP1
• Gene ID (NCBI): 10605
• RRID: AB_2160391
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

In initiation of translation in eukaryotes, binding of the small ribosomal subunit to mRNA requires recognition of the 5-prime cap structure by the cap-binding complex eIF4F. eIF4F consists of eIF4E, eIF4A, and eIF4G. Translation initiation is further regulated by the mRNA 3-prime poly(A) tail and the poly(A)-binding protein (PABC1). PAIP1 interacts with PABC1 and some eIF4 complexes [PMID:9548260]. It is a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs, and associates with EIF4A and PABPC1 may potentiate contacts between mRNA termini [PMID:11051545].

Publications

Species	Application	Title
human	WB	Biochem J; Biological Insights into the Expression of Translation Initiation Factors from Recombinant CHOK1SV Cell Lines and their Relationship to Enhanced Productivity.; Authors - Emma Jane Mead
human	WB,IHC,IF	Biol Reprod; Murine PAIP1 stimulates translation of spermiogenic mRNAs stored by YBX2 via its interaction with YBX2.; Authors - Yue He
human	WB,IHC	Hum Pathol; Paip1 predicts poor prognosis and promotes tumor progression through AKT/GSK-3β pathway in lung adenocarcinoma.; Authors - Yixuan Wang ; KD Validated
human	WB,IF	Exp Cell Res; Role of Paip1 on angiogenesis and invasion in pancreatic cancer.; Authors - Hongjian Guan ; KD Validated
human	WB,IHC	Cancer Res Treat; Paip1 Indicated Poor Prognosis in Cervical Cancer and Promoted Cervical Carcinogenesis.; Authors - Nan Li ; KD Validated
human	WB	Arch Biochem Biophys; YAP1-activated ZNF131 promotes hepatocellular carcinoma cell proliferation through transcriptional regulation of PAIP1; Authors - Guozhi Yin