PAI-1 Monoclonal antibody, PBS Only

PAI-1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H4A5

Cat no : 66261-1-PBS

Synonyms

SERPINE1, SERPINE1, PAI-1, Plasminogen activator inhibitor 1, PAI1, PAI 1

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Validation Data Gallery

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  WB analysis of human peripheral blood platelets using 66261-1-Ig (same clone as 66261-1-PBS)

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 66261-1-Ig (same clone as 66261-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig serum using 66261-1-Ig (same clone as 66261-1-PBS)

  pig serum were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 66261-1-Ig (same clone as 66261-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-251 using 66261-1-Ig (same clone as 66261-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HuH-7 using 66261-1-Ig (same clone as 66261-1-PBS)

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66261-1-Ig (same clone as 66261-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66261-1-Ig (PAI-1 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66261-1-Ig (same clone as 66261-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66261-1-Ig (PAI-1 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 66261-1-Ig (same clone as 66261-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66261-1-Ig (PAI-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 66261-1-Ig (same clone as 66261-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66261-1-Ig (PAI-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66261-1-Ig (same clone as 66261-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PAI-1 antibody (66261-1-Ig, Clone: 1H4A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

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Product Information

66261-1-PBS targets PAI-1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, pig samples.

• Tested Reactivity: human, mouse, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PAI-1 fusion protein Ag22411
• Full Name: serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
• Calculated Molecular Weight: 402 aa, 45 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC010860
• Gene Symbol: PAI-1
• Gene ID (NCBI): 5054
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SERPINE1, also named Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tissue plasminogen activator (tPA) and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. The mature 379-residue protein had a calculated unglycosylated molecular mass of 42.7 kD. Three putative glycosylation sites were found.