PAH Polyclonal antibody

PAH Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 16347-1-AP

Synonyms

PAH, PH, Phe 4 monooxygenase, Phenylalanine 4 hydroxylase, phenylalanine hydroxylase, PKU, PKU1

---

Validation Data Gallery

• 

  WB analysis of HepG2 using 16347-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16347-1-AP (PAH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16347-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16347-1-AP (PAH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 16347-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16347-1-AP (PAH Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 16347-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16347-1-AP (PAH Antibody) at dilution of 1:200 (under 10x lens).

---

Tested Applications

• Positive WB detected in: HepG2 cells, mouse kidney tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

16347-1-AP targets PAH in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PAH fusion protein Ag9541
• Full Name: phenylalanine hydroxylase
• Calculated Molecular Weight: 452 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC026251
• Gene Symbol: PAH
• Gene ID (NCBI): 5053
• RRID: AB_2878247
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAH, also known as PH, belongs to the aromatic amino acid hydroxylase (AAAH) enzyme family. PAH catalyzes the conversion of L-phenylalanine (L-Phe) to L-tyrosine (L-Tyr) by para-hydroxylation of the aromatic side chain. PAH is primarily present in the liver, where removal of excess L-Phe prevents the neurotoxic effect of hyperphenylalaninemia (HPA). The calculated molecular weight of PAH is 52 kDa (PMID: 23457044).

Publications

Species	Application	Title
mouse	WB	Pharmacol Res; Medioresinol as a novel PGC-1α activator prevents pyroptosis of endothelial cells in ischemic stroke through PPARα-GOT1 axis.; Authors - Yunjie Wang
mouse	WB	Life Sci Alliance; A phosphoproteomic approach reveals that PKD3 controls PKA-mediated glucose and tyrosine metabolism.; Authors - Angel Loza-Valdes
human	WB	Adv Sci (Weinh); A Metabolic Reprogramming Amino Acid Polymer as an Immunosurveillance Activator and Leukemia Targeting Drug Carrier for T‐Cell Acute Lymphoblastic Leukemia; Authors - Changzheng Li
human	IHC	Arthritis Res Ther; Investigation of ferroptosis-associated molecular subtypes and immunological characteristics in lupus nephritis based on artificial neural network learning; Authors - Li Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Angel (Verified Customer) (11-27-2020)

This antibody detects PAH in liver tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Liver tissue

PAH Antibody Western Blot validation (1:1000 dilution) in Liver tissue (Cat no:16347-1-AP)