PADI2 Polyclonal antibody

• Featured Product
• KD/KO Validated

PADI2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IF-P, IP, COIP, ELISA, Blocking assay

Conjugate

Unconjugated

Publications

57

Cat no : 12110-1-AP

Synonyms

PADI2 protein, PAD-H19, PAD2, PAD H19, KIAA0994

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Validation Data Gallery

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  WB analysis using 12110-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12110-1-AP (PADI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12110-1-AP

  WB result of PADI2 antibody (12110-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PADI2 transfected MCF-7 cells.

• 

  WB analysis of MCF-7 using 12110-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 12110-1-AP (PADI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12110-1-AP

  IP result of anti-PADI2 (IP:12110-1-AP, 3ug; Detection:12110-1-AP 1:300) with mouse brain tissue lysate 3600ug.

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  IHC staining of human breast cancer using 12110-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12110-1-AP (PADI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12110-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12110-1-AP (PADI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human breast cancer using 12110-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 12110-1-AP (PADI2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 12110-1-AP

  Immunofluorescent analysis of HeLa cells using 12110-1-AP (PADI2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of BxPC-3 using 12110-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 12110-1-AP (PADI2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, MCF-7 cells, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human breast cancer tissue
• Positive IF/ICC detected in: BxPC-3 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 12 publications below
• WB: See 36 publications below
• IHC: See 13 publications below
• IF: See 21 publications below
• FC: See 2 publications below
• CoIP: See 1 publications below

Product Information

12110-1-AP targets PADI2 in WB, IHC, IF/ICC, IF-P, IP, COIP, ELISA, Blocking assay applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PADI2 fusion protein Ag2755
• Full Name: peptidyl arginine deiminase, type II
• Calculated Molecular Weight: 665 aa, 75 kDa
• Observed Molecular Weight: 70-75 kDa, 50 kDa
• GenBank Accession Number: BC009701
• Gene Symbol: PADI2
• Gene ID (NCBI): 11240
• RRID: AB_2159475
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PADI2, also named as KIAA0994, PDI2, PAD-H19, and PAD2(Peptidylarginine deiminase II ), belongs to the protein arginine deiminase family. It catalyzes the deimination of arginine residues of proteins. PADI2 may play a regulatory role in the expression of lactation-related genes via histone citrullination during diestrus (PMID:20668670). PADI2 has two isoforms with MW 75 kDa and 49 kDa.

Publications

Species	Application	Title
human	WB,IF	Sci Transl Med; Neutrophil extracellular traps, B cells, and type I interferons contribute to immune dysregulation in hidradenitis suppurativa.; Authors - Angel S Byrd
human	WB	Mol Cell; Arginine Citrullination at the C-Terminal Domain Controls RNA Polymerase II Transcription.; Authors - Priyanka Sharma ; KD Validated
mouse	WB,FC	J Immunother Cancer; PAD-2-mediated citrullination of nucleophosmin provides an effective target for tumor immunotherapy.; Authors - Ruhul H Choudhury ; KO Validated
mouse	WB,IF	Angiogenesis; Angiogenic responses in a 3D micro-engineered environment of primary endothelial cells and pericytes.; Authors - Jing Bai ; KD Validated
human	WB	Proc Natl Acad Sci U S A; Peptidylarginine deiminase 2-catalyzed histone H3 arginine 26 citrullination facilitates estrogen receptor α target gene activation.; Authors - Zhang Xuesen X
human	WB	Diabetes; Modifying Enzymes are Elicited by ER Stress, Generating Epitopes that are Selectively Recognized by CD4+ T Cells in Patients With Type 1 Diabetes.; Authors - Meghan L Marre

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