PACT Polyclonal antibody

• Featured Product
• KD/KO Validated

PACT Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28636-1-AP

Synonyms

DYT16, HSD 14, HSD14, PACT, PKR associated protein X, PKR associating protein X, PRKRA, RAX

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Validation Data Gallery

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  WB analysis using 28636-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28636-1-AP (PACT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 28636-1-AP

  WB result of PACT antibody (28636-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PACT transfected HeLa cells.

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  WB analysis of MCF-7 using 28636-1-AP

  WB result of PACT antibody (28636-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PACT transfected MCF-7 cells.

• 

  WB analysis using 28636-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28636-1-AP (PRKRA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, mouse testis tissue, MCF-7 cells, rat restis tissue, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28636-1-AP targets PACT in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PACT fusion protein Ag29784
• Full Name: protein kinase, interferon-inducible double stranded RNA dependent activator
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC009470
• Gene Symbol: PRKRA
• Gene ID (NCBI): 8575
• RRID: AB_2918186
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRKRA (PKR-associated protein X) is also named as PACT, RAX, HSD-14 and HSD14. PRKRA was significantly overexpressed in PC tissues and promoted the proliferation, migration, and chemoresistance of PC cells (PMID: 37484321). PRKRA activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. PRKRA has been shown to interact with DICER1. DICER1 and PRKRA expression in normal colon was limited to mucosal epithelium and showed similar cytoplasmic expression (PMID: 37484321).