PACS2 Polyclonal antibody

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  WB analysis of HCT 116 using 30172-1-AP

  WB result of PACS2 antibody (30172-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PACS2 transfected HCT 116 cells.

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  WB analysis using 30172-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30172-1-AP (PACS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30172-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30172-1-AP (PACS2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HCT 116 using 30172-1-AP

  IP result of anti-PACS2 (IP:30172-1-AP, 4ug; Detection:30172-1-AP 1:1500) with HCT 116 cells lysate 2440 ug.

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  IF Staining of HCT 116 using 30172-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using PACS2 antibody (30172-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

PACS2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 30172-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IF/ICC, IP, ELISA

Synonyms

FLJ25488, KIAA0602, PACS 2, PACS1 like protein, PACS1L, PACS2

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Tested Applications

• Positive WB detected in: HCT 116 cells, NIH/3T3 cells, HuH-7 cells
• Positive IP detected in: HCT 116 cells
• Positive IF/ICC detected in: HCT 116 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30172-1-AP targets PACS2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PACS2 fusion protein Ag32902
• Full Name: phosphofurin acidic cluster sorting protein 2
• Calculated Molecular Weight: 98kd
• Observed Molecular Weight: 100-130 kDa
• GenBank Accession Number: NM_001100913
• Gene Symbol: PACS2
• Gene ID (NCBI): 23241
• RRID: AB_3086254
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86VP3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PACS2, also named PACS1L or KIAA0602, is one of PACS (phosphofurin acidic cluster sorting) proteins which are one such family of multifunctional membrane traffic regulators that mediate organ homoeostasis and have important roles in diverse pathologies and disease states. PACS2 is a multifunctional sorting protein that controls the endoplasmic reticulum (ER)-mitochondria communication, ER homeostasis, and apoptosis. PACS2 may be involved in ion channel trafficking, directing acidic cluster-containing ion channels to distinct subcellular compartments.