PABPC4 Polyclonal antibody

• Featured Product
• KD/KO Validated

PABPC4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (1)

Cat no : 14960-1-AP

Synonyms

Inducible poly(A)-binding protein, APP-1, APP1, APP 1, Activated-platelet protein 1

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Validation Data Gallery

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  WB analysis using 14960-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14960-1-AP (PABPC4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 14960-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14960-1-AP (PABPC4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14960-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14960-1-AP (PABPC4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14960-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14960-1-AP (PABPC4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 14960-1-AP

  IP result of anti-PABPC4 (IP:14960-1-AP, 4ug; Detection:14960-1-AP 1:500) with mouse heart tissue lysate 4000ug.

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  IHC staining of mouse skeletal muscle using 14960-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14960-1-AP (PABPC4 antibody) at dilution of 1:500 (under 10x lens).

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  IHC staining of mouse skeletal muscle using 14960-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14960-1-AP (PABPC4 antibody) at dilution of 1:500 (under 40x lens).

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  IF Staining of HepG2 using 14960-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PABPC4 antibody (14960-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 14960-1-AP

  Immunofluorescent analysis of Hela cells, using PABPC4 antibody 14960-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse heart tissue, human brain tissue, Jurkat cells
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Product Information

14960-1-AP targets PABPC4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PABPC4 fusion protein Ag6813
• Full Name: poly(A) binding protein, cytoplasmic 4 (inducible form)
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70-71 kDa
• GenBank Accession Number: BC065540
• Gene Symbol: PABPC4
• Gene ID (NCBI): 8761
• RRID: AB_2156897
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IP	Nucleic Acids Res; Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.; Authors - Evan P Booy
human	WB,IHC,IP	EMBO Rep; LINC00493-encoded microprotein SMIM26 exerts anti-metastatic activity in renal cell carcinoma; Authors - Kun Meng ; KD Validated
human	WB	J Immunol; HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins.; Authors - Adi Reches ; KD Validated
mouse		Nat Struct Mol Biol; Poised PABP-RNA hubs implement signal-dependent mRNA decay in development; Authors - Miha Modic
human	WB	BMC Pregnancy Childbirth; Screening of differentially expressed proteins from syncytiotrophoblast for severe early-onset preeclampsia in women with gestational diabetes mellitus using tandem mass tag quantitative proteomics.; Authors - Xiaotong Sun
human	WB	PLoS Pathog; Elucidation of TRIM25 ubiquitination targets involved in diverse cellular and antiviral processes; Authors - Emily Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Abe (Verified Customer) (04-25-2024)

Worked well for IF at 1:500 overnight in 4c for RPE-1 cells.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: RPE-1