PABPC1,PABP Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PABPC1,PABP Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D8B5

Cat no : 66809-1-PBS

Synonyms

PAB1, PABP, PABP 1, PABP1, PABPC1, PABPC1,PABP, PABPC2, PABPL1, Poly(A) binding protein 1

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Validation Data Gallery

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  WB analysis using 66809-1-Ig (same clone as 66809-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66809-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66809-1-Ig (same clone as 66809-1-PBS)

  WB result of PABPC1,PABP antibody (66809-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PABPC1,PABP transfected HeLa cells. This data was developed using the same antibody clone with 66809-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 66809-1-Ig (same clone as 66809-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66809-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 66809-1-Ig (same clone as 66809-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66809-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66809-1-Ig (same clone as 66809-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66809-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 66809-1-Ig (same clone as 66809-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66809-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 66809-1-Ig (same clone as 66809-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66809-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 66809-1-Ig (same clone as 66809-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66809-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 66809-1-Ig (same clone as 66809-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66809-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 66809-1-Ig (same clone as 66809-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66809-1-PBS in a different storage buffer formulation.

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Product Information

66809-1-PBS targets PABPC1,PABP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PABPC1,PABP fusion protein Ag27860
• Full Name: poly(A) binding protein, cytoplasmic 1
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 71 kDa
• GenBank Accession Number: BC015958
• Gene Symbol: PABPC1
• Gene ID (NCBI): 26986
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation [PMID: 21989405]. Polyadenylate-binding protein 1 (PABPC1) is a cytoplasmic-nuclear shuttling protein important for protein translation initiation, and both RNA processing and stability. In the cytoplasm, PABPC1 binds to the 3' poly(A) tail of eukaryotic mRNAs through its RNA-recognition motifs (RRM) and interacts with the N-terminus of eIF4G, part of the eIF4F complex associated with the 5' cap structure [PMID:20009508, 17381337].