PA2G4 Polyclonal antibody

• Featured Product
• KD/KO Validated

PA2G4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 15348-1-AP

Synonyms

hG4-1, HG4 1, ErbB3-binding protein 1, ErbB3 binding protein 1, EBP1

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 15348-1-AP

  WB result of PA2G4 antibody (15348-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PA2G4 transfected HEK-293 cells.

• 

  WB analysis using 15348-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15348-1-AP (PA2G4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 15348-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 15348-1-AP (PA2G4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 15348-1-AP

  IP result of anti-PA2G4 (IP:15348-1-AP, 4ug; Detection:15348-1-AP 1:1000) with Jurkat cells lysate 3000ug.

• 

  IHC staining of human breast cancer using 15348-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15348-1-AP (PA2G4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15348-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15348-1-AP (PA2G4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15348-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PA2G4 antibody (15348-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

---

Tested Applications

• Positive WB detected in: HEK-293T cells, HEK-293 cells, rat spleen tissue, HL-60 cells, HeLa cells, Jurkat cells, MCF-7 cells, PC-3 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Product Information

15348-1-AP targets PA2G4 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PA2G4 fusion protein Ag7587
• Full Name: proliferation-associated 2G4, 38kDa
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 42 kDa, 48 kDa
• GenBank Accession Number: BC001951
• Gene Symbol: PA2G4
• Gene ID (NCBI): 5036
• RRID: AB_2156602
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PA2G4 (EBP1), first identified as an ErbB3 binding protein, is a 38 kDa protein that is widely expressed in cultured cells and tissues. The presence of several functional domains such as a nuclear localization sequence (NLS), LxxLL and LxCxE suggests the involvement of Ebp1 in cell signaling pathways and gene transcription regulation. This antibody is a rabbit polyclonal antibody raised against full length PA2G4 of human origin.

Publications

Species	Application	Title
human	WB,IHC,IF,RIP	Cell Biosci; PA2G4 promotes the metastasis of hepatocellular carcinoma by stabilizing FYN mRNA in a YTHDF2-dependent manner.; Authors - Sheng Sun ; KD Validated
human	WB,IHC	FASEB J; Evidence for a fragile X messenger ribonucleoprotein 1 (FMR1) mRNA gain-of-function toxicity mechanism contributing to the pathogenesis of fragile X-associated premature ovarian insufficiency; Authors - Roseanne Rosario
		Methods Enzymol; Selective ribosome profiling as a tool to study interactions of translating ribosomes in mammalian cells; Authors - Manuel Günnigmann
human	WB,IF,IP	J Dent Sci; Proliferation-associated 2G4 P48 is stabilized by malignant T-cell amplified sequence 1 and promotes the proliferation of head and neck squamous cell carcinoma; Authors - Legang Sun
human	WB	Nat Commun; Orthogonal proteogenomic analysis identifies the druggable PA2G4-MYC axis in 3q26 AML; Authors - Matteo Marchesini
human	IHC	Appl Immunohistochem Mol Morphol; Increased PA2G4 Expression Is an Unfavorable Factor in Nasopharyngeal Carcinoma; Authors - Yan Xu