P85a; PIK3R1 Polyclonal antibody

P85a; PIK3R1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30092-1-AP

Synonyms

GRB1, p85, p85 ALPHA, PI3 kinase subunit p85 alpha, PI3K regulatory subunit alpha, PIK3R1

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Validation Data Gallery

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  WB analysis using 30092-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30092-1-AP (P85a; PIK3R1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30092-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30092-1-AP (P85a; PIK3R1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, HepG2 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30092-1-AP targets P85a; PIK3R1 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC030815
• Gene Symbol: PI3 Kinase p85 Alpha
• Gene ID (NCBI): 5295
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.