P5CS Monoclonal antibody

P5CS Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3B1E11

Cat no : 68184-1-Ig

Synonyms

ALDH18A1, Gamma-glutamyl phosphate reductase, Gamma-glutamyl kinase, Delta-1-pyrroline-5-carboxylate synthase, Aldehyde dehydrogenase family 18 member A1

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Validation Data Gallery

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  WB analysis using 68184-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68184-1-Ig (P5CS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of mouse colon using 68184-1-Ig

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 68184-1-Ig (P5CS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat colon using 68184-1-Ig

  rat colon tissue were subjected to SDS PAGE followed by western blot with 68184-1-Ig (P5CS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: U2OS cells, mouse colon tissue, rat colon tissue, A549 cells, MCF-7 cells, HeLa cells, LNCaP cells, HepG2 cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

68184-1-Ig targets P5CS in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: P5CS fusion protein Ag12100
• Full Name: aldehyde dehydrogenase 18 family, member A1
• Calculated Molecular Weight: 795 aa, 87 kDa
• Observed Molecular Weight: 87 kDa
• GenBank Accession Number: BC106054
• Gene Symbol: P5CS
• Gene ID (NCBI): 5832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Delta 1-pyrroline-5-carboxylate synthetase (P5CS), also named as GSAS, P5CS, PYCS, catalyzes the ATP and the NAD(P)H-dependent conversion of L-glutamate to glutamic gamma-semialdehyde (GSA) which is the metabolic precursor for proline biosynthesis(PMID:8761662). This protein includes 2 domains: glutamate 5-kinase and gamma-glutamyl phosphate reductase. In the N-terminal section, it belongs to the glutamate 5-kinase family and in the C-terminal section, it belongs to the gamma-glutamyl phosphate reductase family. Defects in ALDH18A1 are the cause of cutis laxa, autosomal recessive, type 3A (ARCL3A)(PMID:18478038). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	IF	Nature; Cellular ATP demand creates metabolically distinct subpopulations of mitochondria; Authors - Keun Woo Ryu