DDX6 Polyclonal antibody

• Featured Product
• KD/KO Validated

DDX6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

12

Reviews (1)

Cat no : 14632-1-AP

Synonyms

RCK, Probable ATP-dependent RNA helicase DDX6, P54, HLR2, EC:3.6.4.13

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Validation Data Gallery

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  WB analysis using 14632-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14632-1-AP (DDX6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 14632-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14632-1-AP (DDX6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14632-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14632-1-AP (DDX6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 14632-1-AP

  IP result of anti-DDX6 (IP:14632-1-AP, 4ug; Detection:14632-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IHC staining of human breast cancer using 14632-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14632-1-AP (DDX6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 14632-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14632-1-AP (DDX6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14632-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14632-1-AP (DDX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14632-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14632-1-AP (DDX6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of hTERT-RPE1 using 14632-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using DDX6 antibody (14632-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 14632-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DDX6 antibody (14632-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, C2C12 cells, mouse testis tissue, rat testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human breast cancer tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IF: See 5 publications below

Product Information

14632-1-AP targets DDX6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDX6 fusion protein Ag6211
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC065007
• Gene Symbol: DDX6
• Gene ID (NCBI): 1656
• RRID: AB_2091264
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

P54, also named as DDX6, HLR2, and RCK, is a 483 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and belongs to the DEAD box helicase family. DDX6/DHH1 subfamily. P54 is abundantly expressed in most tissues. In the process of mRNA degradation, P54 may play a role in mRNA decapping. P54 is involved in the replication of hepatitis C virus genomes in hepatocytes and in tumourigenesis of hepatocellular carcinomas.

Publications

Species	Application	Title
mouse	WB,IF	J Genet Genomics; LSM14B coordinates protein component expression in the P-body and controls oocyte maturation; Authors - Huiru Zhang
human	WB,IF	iScience; The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complex.; Authors - Limei Zou
mouse	WB	Biol Reprod; Translational Activation of Developmental Messenger RNAs During Neonatal Mouse Testis Development.; Authors - Chappell Vesna A VA
human	WB	Biochem Biophys Res Commun; DDX6 is involved in the pathogenesis of inflammatory diseases via NF-κB activation; Authors - Seiichiro Naito ; KD Validated
human	WB	EMBO J; Energy stress promotes P-bodies formation via lysine-63-linked polyubiquitination of HAX1; Authors - Wanqi Zhan
mouse	IF	BMC Bioinformatics; SpotitPy: a semi-automated tool for object-based co-localization of fluorescent labels in microscopy images; Authors - Alexia Akalestou-Clocher

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Roy (Verified Customer) (06-13-2024)

The antibody worked well to detect DDX6 in HeLa cells by WB (1/1000 dilution, Overnight incubation at 4°C) and by Immunofluorescence to observe P-Bodies (1h incubation at RT - 1/500).

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa