Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, C2C12 cells, mouse testis tissue, rat testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human breast cancer tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14632-1-AP targets DDX6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6211 Product name: Recombinant human P54 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 134-483 aa of BC065007 Sequence: RDILARAKNGTGKSGAYLIPLLERLDLKKDNIQAMVIVPTRELALQVSQICIQVSKHMGGAKVMATTGGTNLRDDIMRLDDTVHVVIATPGRILDLIKKGVAKVDHVQMIVLDEADKLLSQDFVQIMEDIILTLPKNRQILLYSATFPLSVQKFMNSHLQKPYEINLMEELTLKGVTQYYAYVTERQKVHCLNTLFSRLQINQSIIFCNSSQRVELLAKKISQLGYSCFYIHAKMRQEHRNRVFHDFRNGLCRNLVCTDLFTRGIDIQAVNVVINFDFPKLAETYLHRIGRSGRFGHLGLAINLITYDDRFNLKSIEEQLGTEIKPIPSNIDKSLYVAEYHSEPVEDEKP Predict reactive species |
| Full Name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 |
| Calculated Molecular Weight | 54 kDa |
| Observed Molecular Weight | 54 kDa |
| GenBank Accession Number | BC065007 |
| Gene Symbol | DDX6 |
| Gene ID (NCBI) | 1656 |
| RRID | AB_2091264 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P26196 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DDX6, also named as P54, HLR2, and RCK, is a 483 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and belongs to the DEAD box helicase family (PMID: 22022269). P54 is abundantly expressed in most tissues, in gastric cancer, DDX6 positively regulated the expression of HER2 and FGFR2 and promoted the process of c-Myc (PMID: 29314290). In the process of mRNA degradation, P54 may play a role in mRNA decapping. P54 is involved in the replication of hepatitis C virus genomes in hepatocytes and in tumourigenesis of hepatocellular carcinomas (PMID: 12823589).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DDX6 antibody 14632-1-AP | Download protocol |
| IHC protocol for DDX6 antibody 14632-1-AP | Download protocol |
| IP protocol for DDX6 antibody 14632-1-AP | Download protocol |
| WB protocol for DDX6 antibody 14632-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
DDX6 Polyclonal antibody (Cat# 14632-1-AP) has 22 citations spanning 18 journals including Nature Cell Biology, Nature Communications, Science Advances, EMBO Journal, Advanced Science, Nucleic Acids Research, Journal of Cell Biology, and iScience. Research fields cover P-body biology, mRNA regulation and translation, stress granule dynamics, oocyte maturation, cancer and chemoresistance, RNA-binding proteins, inflammatory disease, stem cell differentiation, and neurodegeneration. Applications cited include WB, IF, and IP in human and mouse models.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Cyclophilin A supports translation of intrinsically disordered proteins and affects haematopoietic stem cell ageing | ||
Nat Commun DDX6 undergoes phase separation to modulate metabolic plasticity and chemoresistance.
| ||
Nat Commun Systematic characterization of the composition and dynamics of processing body-associated mRNAs. | ||
Nucleic Acids Res LSM14B is essential for oocyte meiotic maturation by regulating maternal mRNA storage and clearance | ||
Adv Sci (Weinh) Fusidic Acid Reverses Chemoresistance in Breast Cancer via Targeting DDX6 to Downregulate GSK-3β/β-Catenin Signaling | ||
Adv Sci (Weinh) Primate-Specific DAZ Regulates Translation of Cell Proliferation-Related mRNAs and is Essential for Maintenance of Spermatogonia |
Reviews
What customers say
One reviewer (Matkovic, 2024) gave a 5-star rating for this DDX6 antibody. They confirmed it worked well for Western Blot at 1/1000 dilution with overnight 4°C incubation on HeLa cells, and for Immunofluorescence to visualize P-Bodies at 1/500 dilution with 1-hour RT incubation. Overall, the antibody performed reliably across both applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Roy (Verified Customer) (06-13-2024) | The antibody worked well to detect DDX6 in HeLa cells by WB (1/1000 dilution, Overnight incubation at 4°C) and by Immunofluorescence to observe P-Bodies (1h incubation at RT - 1/500).
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