P4HA1 Polyclonal antibody

• Featured Product
• KD/KO Validated

P4HA1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

23

Cat no : 12658-1-AP

Synonyms

Prolyl 4-hydroxylase subunit alpha-1, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1, P4HA, EC:1.14.11.2

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Validation Data Gallery

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  WB analysis using 12658-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12658-1-AP (P4HA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skin using 12658-1-AP

  rat skin tissue were subjected to SDS PAGE followed by western blot with 12658-1-AP (P4HA1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 12658-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 12658-1-AP (P4HA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 12658-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12658-1-AP (P4HA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 12658-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12658-1-AP (P4HA1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 12658-1-AP

  IP result of anti-P4HA1 (IP:12658-1-AP, 3ug; Detection:12658-1-AP 1:600) with A431 cells lysate 3300ug.

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  IHC staining of human pancreas cancer using 12658-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12658-1-AP (P4HA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 12658-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12658-1-AP (P4HA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12658-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12658-1-AP (P4HA1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 12658-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12658-1-AP (P4HA1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A431 using 12658-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A431 cells using 12658-1-AP (P4HA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, NIH/3T3 cells, human heart tissue, rat skin tissue, HT-1080 cells, BGC-823 cells, PC-3 cells
• Positive IP detected in: A431 cells
• Positive IHC detected in: human pancreas cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 9 publications below
• WB: See 18 publications below
• IHC: See 10 publications below
• IF: See 2 publications below

Product Information

12658-1-AP targets P4HA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: P4HA1 fusion protein Ag3292
• Full Name: prolyl 4-hydroxylase, alpha polypeptide I
• Calculated Molecular Weight: 534 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC034998
• Gene Symbol: P4HA1
• Gene ID (NCBI): 5033
• RRID: AB_2283162
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

P4HA1 (Prolyl 4-hydroxylase subunit alpha-1) is also known as P4HA and belongs to the P4HA family, which plays a central role in collagen synthesis. P4HA1 catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. The gene encodes a polypeptide of 517 amino acid residues and a signal peptide of 17 amino acids and the full length protein has two glycosylation sites (uniprot). It can exist as a heteromer, dimer or tetramer (refer to: GENATLAS). P4HA1 has some isoforms with the MW 61-63 kDa and 57-59 kDa.

Publications

Species	Application	Title
human	WB	Nature; Cotranslational prolyl hydroxylation is essential for flavivirus biogenesis.; Authors - Ranen Aviner
mouse,rat	WB	Cell Death Differ; Developmental growth plate cartilage formation suppressed by artificial light at night via inhibiting BMAL1-driven collagen hydroxylation; Authors - Guangjin Chen
human,mouse	WB	J Exp Med; IL-17-induced HIF1α drives resistance to anti-PD-L1 via fibroblast-mediated immune exclusion.; Authors - Xing Chen
human	WB, IHC	Oncotarget; The miR-124-prolyl hydroxylase P4HA1-MMP1 axis plays a critical role in prostate cancer progression.; Authors - Balabhadrapatruni V S K Chakravarthi ; KD Validated
human	WB,IHC,IF	Mol Oncol; Prolyl 4-hydroxylase subunit alpha 1 (P4HA1) is a biomarker of poor prognosis in primary melanomas, and its depletion inhibits melanoma cell invasion and disrupts tumor blood vessel walls.; Authors - Johanna Eriksson ; KD Validated
human	WB	Aging (Albany NY); Identification of a novel glycolysis-related gene signature for predicting the prognosis of osteosarcoma patients.; Authors - Mengkai Yang ; KD Validated