P2RY1 Monoclonal antibody, PBS Only

P2RY1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Mouse, Rat, Rabbit

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1A1F3

Cat no : 67654-1-PBS

Synonyms

ATP receptor, P2RY1, P2Y purinoceptor 1, P2Y1, Purinergic receptor

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Validation Data Gallery

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  WB analysis using 67654-1-Ig (same clone as 67654-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67654-1-Ig (P2RY1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67654-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67654-1-Ig (same clone as 67654-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67654-1-Ig (P2RY1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67654-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67654-1-Ig (same clone as 67654-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67654-1-Ig (P2RY1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67654-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 67654-1-Ig (same clone as 67654-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67654-1-Ig (P2RY1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67654-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 67654-1-Ig (same clone as 67654-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67654-1-Ig (P2RY1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67654-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 67654-1-Ig (same clone as 67654-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using P2RY1 antibody (67654-1-Ig, Clone: 1A1F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67654-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 67654-1-Ig (same clone as 67654-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using P2RY1 antibody (67654-1-Ig, Clone: 1A1F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67654-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 67654-1-Ig (same clone as 67654-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using P2RY1 antibody (67654-1-Ig, Clone: 1A1F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67654-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 67654-1-Ig (same clone as 67654-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using P2RY1 antibody (67654-1-Ig, Clone: 1A1F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67654-1-PBS in a different storage buffer formulation.

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Product Information

67654-1-PBS targets P2RY1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat, Rabbit samples.

• Tested Reactivity: Human, Pig, Mouse, Rat, Rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: P2RY1 fusion protein Ag12762
• Full Name: purinergic receptor P2Y, G-protein coupled, 1
• Calculated Molecular Weight: 373 aa, 42 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC074785
• Gene Symbol: P2RY1
• Gene ID (NCBI): 5028
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.