P2RX4 Monoclonal antibody, PBS Only

P2RX4 Monoclonal Antibody for WB, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, pig, rat

Applications

WB, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A5A6

Cat no : 66416-1-PBS

Synonyms

ATP receptor, P2RX4, P2X purinoceptor 4, P2X4, P2X4R, Purinergic receptor

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Validation Data Gallery

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  WB analysis using 66416-1-Ig (same clone as 66416-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66416-1-Ig (P2RX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66416-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66416-1-Ig (same clone as 66416-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66416-1-Ig (P2RX4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66416-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 66416-1-Ig (same clone as 66416-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66416-1-Ig (P2RX4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66416-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66416-1-Ig (same clone as 66416-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66416-1-Ig (P2RX4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66416-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66416-1-Ig (same clone as 66416-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66416-1-Ig (P2RX4 Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66416-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66416-1-Ig (same clone as 66416-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66416-1-Ig (P2RX4 Antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66416-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66416-1-Ig (same clone as 66416-1-PBS)

  1X10^6 HeLa cells were stained with 0.20 ug Anti-Human P2RX4 (66416-1-Ig, Clone:1A5A6) (red) or 0.20 ug isotype control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Cells were fixed with 90% MeOH. This data was developed using the same antibody clone with 66416-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66416-1-PBS targets P2RX4 in WB, FC, IHC, Indirect ELISA applications and shows reactivity with human, mouse, pig, rat samples.

• Tested Reactivity: human, mouse, pig, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: P2RX4 fusion protein Ag4445
• Full Name: purinergic receptor P2X, ligand-gated ion channel, 4
• Calculated Molecular Weight: 388 aa, 43 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC033826
• Gene Symbol: P2RX4
• Gene ID (NCBI): 5025
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Nucleotides, the structural subunits of the nucleic acids, are also important extracellular signaling molecules. P2 receptors are a family of cell surface receptors that mediate a wide variety of physiologic effects in response to extracellular nucleotides. These receptors fall into two classes: P2X receptors, which are ligand-gated ion channels that mediate calcium and potassium fluxes in response to ATP, and P2Y receptors, which are G protein-coupled receptors (GPCRs). P2RX4 (P2X purinoceptor 4) is a receptor for ATP acting as a ligand gated ion channel. The calculated molecular weight of P2RX4 is 43 kDa, larger apparent molecular weights of 50-80 kDa have been reported, possibly due to post-translational glycosylation (PMID: 24040145, 15262999; 29382907; 12566439).