P27; KIP1 Monoclonal antibody, PBS Only

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  WB analysis of Jurkat using 67355-1-Ig (same clone as 67355-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67355-1-Ig (P27; KIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67355-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 67355-1-Ig (same clone as 67355-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67355-1-Ig (P27; KIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67355-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 67355-1-Ig (same clone as 67355-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67355-1-Ig (P27; KIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67355-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 67355-1-Ig (same clone as 67355-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67355-1-Ig (P27; KIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67355-1-PBS in a different storage buffer formulation.

P27; KIP1 Monoclonal Antibody for WB, Indirect ELISA

Cat No. 67355-1-PBS

Clone No.3F12C10

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

CDKN1B, CDKN4, KIP1, MEN1B, MEN4, P27, P27; KIP1, P27KIP1

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Product Information

67355-1-PBS targets P27; KIP1 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: P27; KIP1 fusion protein Ag14634
• Full Name: cyclin-dependent kinase inhibitor 1B (p27, Kip1)
• Calculated Molecular Weight: 198 aa, 22 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC001971
• Gene Symbol: p27 Kip1/CDKN1B
• Gene ID (NCBI): 1027
• RRID: AB_2882610
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P46527
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

DKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer.