P27; KIP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

P27; KIP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 26714-1-AP

Synonyms

CDKN1B, P27, MEN1B, KIP1, Cyclin-dependent kinase inhibitor p27

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Validation Data Gallery

• 

  WB analysis of NIH/3T3 using 26714-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 26714-1-AP

  WB result of P27; KIP1 antibody (26714-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P27; KIP1 transfected HeLa cells.

• 

  WB analysis of MCF-7 using 26714-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 26714-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 26714-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 26714-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 26714-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 26714-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 26714-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26714-1-AP (P27; KIP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 26714-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26714-1-AP (P27; KIP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 26714-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26714-1-AP (P27; KIP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 26714-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26714-1-AP (P27; KIP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 26714-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 26714-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 26714-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 26714-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 26714-1-AP (P27; KIP1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 26714-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using P27; KIP1 antibody (26714-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  FC experiment of MCF-7 using 26714-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human P27; KIP1 (26714-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, MCF-7 cells, HeLa cells
• Positive IHC detected in: human gliomas tissue, human tonsillitis tissue, human lung cancer tissue, human breast cancer tissue, human colon cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, MCF-7 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

26714-1-AP targets P27; KIP1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: P27; KIP1 fusion protein Ag25083
• Full Name: cyclin-dependent kinase inhibitor 1B (p27, Kip1)
• Calculated Molecular Weight: 198 aa, 22 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC001971
• Gene Symbol: p27 Kip1/CDKN1B
• Gene ID (NCBI): 1027
• RRID: AB_2880611
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer.

Publications

Species	Application	Title
human	WB	Int J Oncol; Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells.; Authors - Wei Jia
human	WB	Biosci Rep; C1QTNF6 regulates cell proliferation and apoptosis of NSCLC in vitro and in vivo.; Authors - Wei Zhang

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