P21 Polyclonal antibody

P21 Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10240-1-AP

Synonyms

CDKN1A, P21;CDKN1A, CDK-interacting protein 1, MDA-6, Cyclin-dependent kinase inhibitor 1

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Validation Data Gallery

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Published Applications

• WB: See 2 publications below

Product Information

10240-1-AP targets P21 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: P21 fusion protein Ag0308
• Full Name: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
• Calculated Molecular Weight: 21 kDa
• GenBank Accession Number: BC000312
• Gene Symbol: p21
• Gene ID (NCBI): 1026
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

P21 (also known as CIP1, WAF1) is a cyclin-dependent kinase inhibitor. P21 binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of P21 is induced by wild-type but not mutant p53 protein, through which P21 mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. P21 can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. P21 was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.

Publications

Species	Application	Title
human	WB	PLoS One; FLASH knockdown sensitizes cells to Fas-mediated apoptosis via down-regulation of the anti-apoptotic proteins, MCL-1 and Cflip short.; Authors - Chen Song S
human,mouse	WB	Head Neck; TMEM252 inhibits epithelial-mesenchymal transition and progression in papillary thyroid carcinoma by regulating Notch1 expression; Authors - Shuyong Zhang