P16-INK4A Monoclonal antibody, PBS Only

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  WB analysis using 60626-1-Ig (same clone as 60626-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60626-1-Ig (P16-INK4A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60626-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60626-1-Ig (same clone as 60626-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60626-1-Ig (P16-INK4A antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60626-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60626-1-Ig (same clone as 60626-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using P16-INK4A antibody (60626-1-Ig, Clone: 1C9D3 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 60626-1-PBS in a different storage buffer formulation.

P16-INK4A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 60626-1-PBS

Clone No.1C9D3

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

p16, p16 INK4, P16 INK4A, p16-INK4, p16INK4A

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Product Information

60626-1-PBS targets P16-INK4A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: P16-INK4A fusion protein Ag1328
• Full Name: cyclin-dependent kinase inhibitor 2A
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC021998
• Gene Symbol: CDKN2A
• Gene ID (NCBI): 1029
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P42771
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

P16-INK4A is also named as CDKN2A, MLM, Tumor suppressor ARF, Alternative reading frame. The tumor suppressor protein p16Ink4a (encoded from the CDKN2A locus) is often transcriptionally activated in cells undergoing senescence and is one of the main regulators of this program, and it is upregulated in multiple tissues during aging (PMID:17055429). p16-Ink4a is the principal member of the Ink4 family of CDK inhibitors. p16-Ink4a contributes to the regulation of cell cycle progression by inhibiting the S phase. p16Ink4a binds to CDK4/6, inhibiting cyclin D-CDK4/6 complex formation and CDK4/6-mediated phosphorylation of Rb family members. Expression of p16-Ink4a maintains the Rb family members in a hypophosphorylated state, which promotes binding to E2F1 and leads to G1 cell cycle arrest (PMID: 21297668).