Osteoprotegerin/TNFRSF11B Recombinant antibody

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  WB analysis of human milk using 83506-1-RR

  human milk was subjected to SDS PAGE followed by western blot with 83506-1-RR (Osteoprotegerin/TNFRSF11B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat kidney using 83506-1-RR

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 83506-1-RR (Osteoprotegerin/TNFRSF11B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 83506-1-RR

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 83506-1-RR (Osteoprotegerin/TNFRSF11B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83506-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83506-1 against Human Osteoprotegerin/ TNFRSF11B were performed. The affinity constant is below 1 pM.

Osteoprotegerin/TNFRSF11B {ptg:Brand} Recombinant Antibody for WB, IHC, ELISA

Cat No. 83506-1-RR

Clone No.240168E9

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Synonyms

TNFRSF11B, Osteoprotegerin, Osteoclastogenesis inhibitory factor, OPG, OCIF

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Tested Applications

• Positive WB detected in: human milk
• Positive IHC detected in: rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83506-1-RR targets Osteoprotegerin/TNFRSF11B in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Osteoprotegerin/TNFRSF11B fusion protein Ag2139
• Full Name: tumor necrosis factor receptor superfamily, member 11b
• Calculated Molecular Weight: 401 aa, 46 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC030155
• Gene Symbol: TNFRSF11B
• Gene ID (NCBI): 4982
• RRID: AB_3671131
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O00300
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Osteoprotegerin (OPG), also known as tumor necrosis factor receptor superfamily member 11B (TNFRSF11B) and osteoclastogenesis inhibitory factor (OCIF), belongs to the tumor necrosis factor (TNF) receptor superfamily. By binding to RANKL, OPG inhibits osteoclastic activity and promotes bone formation. OPG plays a key role in cell survival by inhibiting TRAIL-induced apoptosis. OPG also is involved in vascular biology, fibrosis, immunity, EMT, and the apoptosis of cancer cells (PMID: 35523775).