ORAI1 Polyclonal antibody

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  WB analysis of human spleen using 14443-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 14443-1-AP (Orai1-Alpha antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 14443-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14443-1-AP (Orai1-Alpha antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 14443-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14443-1-AP (Orai1-Alpha antibody at dilution of 1:50 (under 40x lens).

ORAI1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14443-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ORAI1, ORAI1 L1, ORAI1 L2, Orail L2, TMEM142A

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Tested Applications

• Positive WB detected in: human spleen tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

14443-1-AP targets ORAI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ORAI calcium release-activated calcium modulator 1
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: NM_032790
• Gene Symbol: ORAI1
• Gene ID (NCBI): 84876
• RRID: AB_10639506
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96D31
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a 35-45 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene. This antibody may be used to block the ion currents mediated by Orai1. ORAI1 contains 2 isoforms  produced by alternative initiation(uniprot: Q96D31). Catalog#14443-1-AP only recognises alpha isoform.

Publications

Species	Application	Title
rat	WB	PLoS One; Coupling of store-operated calcium entry to vasoconstriction is acid-sensing ion channel 1a dependent in pulmonary but not mesenteric arteries.; Authors - Selina M Garcia