CoraLite®594-conjugated Occludin Polyclonal antibody

• Featured Product
• KD/KO Validated

Occludin Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Publications

2

Cat no : CL594-13409

Synonyms

occludin, OCLN

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Validation Data Gallery

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  IF Staining of T-47D using CL594-13409

  Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using CoraLite®594 Occludin antibody (CL594-13409) at dilution of 1:100.

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  IF Staining of MCF-7 using CL594-13409

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 Occludin antibody (CL594-13409) at dilution of 1:600.

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Tested Applications

• Positive IF/ICC detected in: MCF-7 cells, T-47D cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below

Product Information

CL594-13409 targets Occludin in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Occludin fusion protein Ag4057
• Full Name: occludin
• Calculated Molecular Weight: 522 aa, 59 kDa
• GenBank Accession Number: BC029886
• Gene Symbol: Occludin
• Gene ID (NCBI): 4950
• RRID: AB_2919795
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

Publications

Species	Application	Title
mouse	IF	J Nanobiotechnology; Orally biomimetic metal-phenolic nanozyme with quadruple safeguards for intestinal homeostasis to ameliorate ulcerative colitis; Authors - Yuanyuan Zhu
mouse	IF	Mucosal Immunol; Stress systems exacerbate the inflammatory response after corneal abrasion in sleep-deprived mice via the IL-17 signaling pathway; Authors - Yunxia Xue