Occludin Recombinant antibody, PBS Only

Occludin Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Pig

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

8B2

Cat no : 80545-1-PBS

Synonyms

OCLN, 8B2

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Validation Data Gallery

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  WB analysis using 80545-1-RR (same clone as 80545-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 80545-1-RR (Occludin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80545-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80545-1-RR (same clone as 80545-1-PBS)

  HaCaT cells were subjected to SDS PAGE followed by western blot with 80545-1-RR (Occludin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80545-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig colon using 80545-1-RR (same clone as 80545-1-PBS)

  pig colon tissue were subjected to SDS PAGE followed by western blot with 80545-1-RR (Occludin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80545-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse colon using 80545-1-RR (same clone as 80545-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 80545-1-RR (Occludin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80545-1-PBS in a different storage buffer formulation.

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  IP experiment of HUVEC using 80545-1-RR (same clone as 80545-1-PBS)

  IP result of anti-Occludin (IP:80545-1-RR, 4ug; Detection:80545-1-RR 1:15000) with HUVEC cells lysate 1410 ug. This data was developed using the same antibody clone with 80545-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 80545-1-RR (same clone as 80545-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Occludin antibody (80545-1-RR, Clone: 8B2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 80545-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80545-1-PBS

  Biolayer interferometry (BLl) kinetic assays of 80545-1-RR against Human Occludin were performed. The affinity constant is 26.5 pM.

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Product Information

80545-1-PBS targets Occludin in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, Mouse, Pig samples.

• Tested Reactivity: Human, Mouse, Pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Occludin fusion protein Ag26173
• Full Name: occludin
• Calculated Molecular Weight: 522 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC029886
• Gene Symbol: Occludin
• Gene ID (NCBI): 4950
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).