Occludin Monoclonal antibody, PBS Only (Capture/Detector)

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  WB analysis using 66378-1-Ig (same clone as 66378-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66378-1-Ig (Occludin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66378-1-Ig (same clone as 66378-1-PBS)

  WB result of Occludin antibody (66378-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Occludin transfected Jurkat cells. This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

• 

  WB analysis of LNCaP using 66378-1-Ig (same clone as 66378-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66378-1-Ig (Occludin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66378-1-Ig (same clone as 66378-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66378-1-Ig (Occludin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig colon using 66378-1-Ig (same clone as 66378-1-PBS)

  pig colon tissue were subjected to SDS PAGE followed by western blot with 66378-1-Ig (Occludin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66378-1-Ig (same clone as 66378-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66378-1-Ig (Occludin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66378-1-Ig (same clone as 66378-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66378-1-Ig (Occludin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66378-1-Ig (same clone as 66378-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66378-1-Ig (Occludin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 66378-1-Ig (same clone as 66378-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66378-1-Ig (Occludin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 66378-1-Ig (same clone as 66378-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66378-1-Ig (Occludin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51160-1

  Cytometric bead array standard curve of MP51160-1, Occludin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66378-2-PBS. Detection antibody: 66378-1-PBS. Standard:Ag4057. Range: 0.098-100 ng/mL.

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  Cytometric bead array standard curve of MP51160-3

  Cytometric bead array standard curve of MP51160-3, Occludin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66378-1-PBS. Detection antibody: 66378-4-PBS. Standard:Ag4057. Range: 0.098-50 ng/mL.

• Featured Product
• KD/KO Validated

Occludin Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Cat No. 66378-1-PBS

Clone No.1D3C4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

OCLN, 1D3C4

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Product Information

66378-1-PBS targets Occludin as part of a matched antibody pair:

MP51160-1: 66378-2-PBS capture and 66378-1-PBS detection (validated in Cytometric bead array)

MP51160-3: 66378-1-PBS capture and 66378-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Occludin fusion protein Ag4057
• Full Name: occludin
• Calculated Molecular Weight: 522 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC029886
• Gene Symbol: Occludin
• Gene ID (NCBI): 4950
• RRID: AB_2881755
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q16625
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).