Validation Data Gallery
|Positive WB detected in||HUVEC cells, HepG2 cells, mouse brain tissue, mouse colon tissue, mouse kidney tissue, mouse liver tissue, rat liver tissue, rat brain tissue|
|Positive IP detected in||HUVEC cells|
|Positive IHC detected in||human colon tissue, human breast cancer tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Caco-2 cells, MCF-7 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:10000|
|Immunofluorescence (IF)||IF : 1:400-1:1600|
|Sample-dependent, check data in validation data gallery|
27260-1-AP targets Occludin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse , rat samples.
|Tested Reactivity||human, mouse , rat|
|Cited Reactivity||human, mouse, rat, rabbit, pig, bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Occludin fusion protein Ag26173|
|Calculated molecular weight||522 aa, 59 kDa|
|Observed molecular weight||59 kDa|
|GenBank accession number||BC029886|
|Gene ID (NCBI)||4950|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).
|Product Specific Protocols|
|WB protocol for Occludin antibody 27260-1-AP||Download protocol|
|IHC protocol for Occludin antibody 27260-1-AP||Download protocol|
|IF protocol for Occludin antibody 27260-1-AP||Download protocol|
|IP protocol for Occludin antibody 27260-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Iram (Verified Customer) (06-13-2022)
Excellent antibody for immunoblotting
Balawant (Verified Customer) (05-08-2022)
This antibody I have used and is working great . 1:2000 dilution is working great for the cell line (Colonic epithelialcellr of human).
Kushal (Verified Customer) (03-14-2022)