Occludin Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Occludin Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13409-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 13409-1-AP (same clone as 13409-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13409-1-AP (Occludin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 13409-1-AP (same clone as 13409-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13409-1-AP (Occludin Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 13409-1-AP (same clone as 13409-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13409-1-AP (OCLN antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 13409-1-AP (same clone as 13409-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 13409-1-AP (OCLN antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 13409-1-AP (same clone as 13409-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13409-1-AP (Occludin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 13409-1-AP (same clone as 13409-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13409-1-AP (Occludin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse liver using 13409-1-AP (same clone as 13409-1-PBS)

  IP result of anti-Occludin (IP:13409-1-AP, 3ug; Detection:13409-1-AP 1:500) with mouse liver tissue lysate 6400ug. This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13409-1-AP (same clone as 13409-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13409-1-AP (Occludin Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13409-1-AP (same clone as 13409-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13409-1-AP (Occludin Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 13409-1-AP (same clone as 13409-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13409-1-AP (Occludin Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 13409-1-AP (same clone as 13409-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13409-1-AP (Occludin Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 13409-1-AP (same clone as 13409-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Occludin antibody (13409-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 13409-1-AP (same clone as 13409-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Occludin antibody (13409-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

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Product Information

13409-1-PBS targets Occludin in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Occludin fusion protein Ag4057
• Full Name: occludin
• Calculated Molecular Weight: 522 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC029886
• Gene Symbol: Occludin
• Gene ID (NCBI): 4950
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).