• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

OXPHOS Cocktail (Human recommended)

Cat no : PK30006

Validation Data Gallery

Filter:
  • Various lysates were subjected to SDS PAGE followed by western blot with PK30006 (OXPHOS Cocktail (Human recommended)) at dilution of 1:4000 incubated at room temperature for 1 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with PK30006 (OXPHOS Cocktail (Human recommended)) at dilution of 1:4000 incubated at room temperature for 1 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 10620-1-AP (SDHB antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 10620-1-AP (SDHB antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 14676-1-AP (ATP5A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 14676-1-AP (ATP5A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 14794-1-AP (NDUFB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 14794-1-AP (NDUFB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 55070-1-AP (MTCO2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 55070-1-AP (MTCO2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

Product Information

OXPHOS (oxidative phosphorylation) requires the transport of electrons to molecular oxygen by the mitochondrial respiratory chain which involves four multi-subunit complexes known as complexes I, II, III, and IV (CI, CII, CIII and CIV) and two mobile electron carriers, coenzyme Q (CoQ) and cytochrome c. The respiratory chain generates a transmembrane proton gradient that is channeled by complex V (also known as ATP synthase, CV) to synthesize ATP.

    This OXPHOS kit contains five Polyclonal antibodies. The kit is suitable for Western Blotting analysis of the relative levels of the 5 OXPHOS complexes in mitochondrial preparations from  human. Individual proteins that need to be tested can be purchased according to the corresponding catalog number.



Cat.No            Name            

Complex                

MW            Size            
Antibodies            14676-1-AP            ATP5A1            Complexes Ⅴ            50-55 kDa            100ul
            
21705-1-AP            UQCRC1            Complexes Ⅲ            45 kDa            
10620-1-AP            SDHB            Complexes Ⅱ            32 kDa            
55070-1-AP            MTCO2            Complexes Ⅳ            23-25 kDa            
14794-1-AP            NDUFB8            ComplexesⅠ            19-22 kDa            


Storage

Store at -20℃. Stable for one year after shipment.

Cited in Article as

PK30006, OXPHOS Cocktail (Human recommended), Proteintech, IL, USA