OXA1L Polyclonal antibody

• Featured Product
• KD/KO Validated

OXA1L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

10

Reviews (1)

Cat no : 21055-1-AP

Synonyms

Oxidase assembly 1-like protein, OXA1-like protein, OXA1

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Validation Data Gallery

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  WB analysis using 21055-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21055-1-AP (OXA1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21055-1-AP

  WB result of OXA1L antibody (21055-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OXA1L transfected HeLa cells.

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  WB analysis of NIH/3T3 using 21055-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21055-1-AP (OXA1L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 21055-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21055-1-AP (OXA1L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 21055-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using OXA1L antibody (21055-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 21055-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 21055-1-AP (OXA1L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, NIH/3T3 cells, L02 cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

21055-1-AP targets OXA1L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OXA1L fusion protein Ag13819
• Full Name: oxidase (cytochrome c) assembly 1-like
• Calculated Molecular Weight: 437 aa, 49 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC001669
• Gene Symbol: OXA1L
• Gene ID (NCBI): 5018
• RRID: AB_10695769
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OXA1L/Oxa1 is a member of the conserved Oxa1/YidC/Alb3 protein family and is required for cytochrome c oxidase assembly. The OXA1L protein was initially identified in yeast proteins involved in the biogenesis of the mitochondrial respiratory chain complexes. It locates in the inner membrane of mitochondria and may be involved in mediating the insertion of proteins from the matrix into the inner membrane.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Proteolytic cleavage of opa1 stimulates mitochondrial inner membrane fusion and couples fusion to oxidative phosphorylation.; Authors - Prashant Mishra
human	WB,IP	Nat Commun; LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding.; Authors - Chun-Shik Shin
human	WB	Am J Hum Genet; Recurrent De Novo Dominant Mutations in SLC25A4 Cause Severe Early-Onset Mitochondrial Disease and Loss of Mitochondrial DNA Copy Number.; Authors - Kyle Thompson
human	WB	EMBO Mol Med; OXA1L mutations cause mitochondrial encephalopathy and a combined oxidative phosphorylation defect.; Authors - Kyle Thompson ; KD Validated
mouse	WB	Aging (Albany NY); Reduced mitochondrial translation prevents diet-induced metabolic dysfunction but not inflammation.; Authors - Kara L Perks
mouse	WB	J Cell Sci; Cardiolipin is required for membrane docking of mitochondrial ribosomes and protein synthesis; Authors - Richard G. Lee

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (04-28-2023)

clean band around the expected size

• Applications: Western Blot