OTUD5 Polyclonal antibody

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  WB analysis using 21002-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 21002-1-AP (OTUD5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 21002-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21002-1-AP (OTUD5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 21002-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using OTUD5 antibody (21002-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

OTUD5 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 21002-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, CoIP, ELISA

Synonyms

OTUD 5, OTU domain-containing protein 5, EC:3.4.19.12, DUBA

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Tested Applications

• Positive WB detected in: HEK-293T cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, NIH/3T3 cells, C6 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

21002-1-AP targets OTUD5 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OTUD5 fusion protein Ag15190
• Full Name: OTU domain containing 5
• Calculated Molecular Weight: 571 aa, 61 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC028225
• Gene Symbol: OTUD5
• Gene ID (NCBI): 55593
• RRID: AB_2878788
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96G74
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The deubiquitinating enzyme OTUD5, also known as deubiquitinating enzyme A (DUBA), is a member of the deubiquitinating enzyme OTU family. By deubiquitinating other proteins, OTUD5 is an important regulator of several physiological processes, such as immune signaling and DNA damage responses. The dysregulation of these processes can increase the risk of associated diseases, including inflammation, cancer and genetic disorders.

Publications

Species	Application	Title
human,mouse	WB,IP,IF	Nat Commun; OTUD5 promotes end-joining of deprotected telomeres by promoting ATM-dependent phosphorylation of KAP1S824; Authors - Shiu Yeung Lam
human,mouse	WB,IF	Adv Sci (Weinh); OTUD5 Protects Dopaminergic Neurons by Promoting the Degradation of α-Synuclein in Parkinson's Disease Model; Authors - Xiaomeng Song
human	WB	Clin Transl Med; p53 inhibits OTUD5 transcription to promote GPX4 degradation and induce ferroptosis in gastric cancer; Authors - Junjing Zhang
human,mouse	WB	Int J Radiat Oncol Biol Phys; USP11 exacerbates radiation induced pneumonitis by activating endothelial cell inflammatory response via OTUD5-STING signaling; Authors - Yiting Tang
human	WB	Carcinogenesis; CacyBP promotes the development of lung adenocarcinoma by regulating OTUD5; Authors - Mixue Bai
mouse	WB,IF,CoIP	Nat Commun; Podocyte OTUD5 alleviates diabetic kidney disease through deubiquitinating TAK1 and reducing podocyte inflammation and injury; Authors - Ying Zhao