OTUD4 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

OTUD4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 25070-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis using 25070-1-AP (same clone as 25070-1-PBS)

  Various lysate were subjected to SDS PAGE followed by western blot with 25070-1-AP (OTUD4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25070-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 25070-1-AP (same clone as 25070-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25070-1-AP (OTUD4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25070-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 25070-1-AP (same clone as 25070-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25070-1-AP (OTUD4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25070-1-PBS in a different storage buffer formulation.

---

Product Information

25070-1-PBS targets OTUD4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OTUD4 fusion protein Ag18401
• Full Name: OTU domain containing 4
• Calculated Molecular Weight: 1113 aa, 124 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC118653
• Gene Symbol: OTUD4
• Gene ID (NCBI): 54726
• RRID: AB_2879882
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

OTUD4, a member of the OTU family, encodes for a protein of 495 amino acids with an OTU domain and functions via their enzymatic deubiquitinating activities. OTUD4 nominally encodes a K48-specific deubiquitinase previously shown to counteract proteasomal degradation of DNA repair proteins. OTUD4 has been identified to be down-regulated in multiple human tumor types, and lower OTUD4 expression indicates a poor prognosis.(PMID: 36411454,PMID: 32184655,PMID: 29395066)