oncostatin M Polyclonal antibody

oncostatin M Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 27792-1-AP

Synonyms

OSM, Oncostatin-M

---

Validation Data Gallery

• 

  WB analysis of MCF-7 using 27792-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27792-1-AP (OSM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 27792-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27792-1-AP (OSM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 27792-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 27792-1-AP (OSM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 27792-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27792-1-AP (oncostatin M antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 27792-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27792-1-AP (oncostatin M antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 27792-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 27792-1-AP (OSM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 27792-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 27792-1-AP (OSM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: MCF-7 cells, Jurkat cells, rat testis tissue
• Positive IHC detected in: human colon cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

27792-1-AP targets oncostatin M in WB, IHC, IF, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: oncostatin M fusion protein Ag27093
• Full Name: oncostatin M
• Calculated Molecular Weight: 252 aa, 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC011589
• Gene Symbol: OSM
• Gene ID (NCBI): 5008
• RRID: AB_2880974
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Oncostatin M (OSM) is a multifunctional cytokine that belongs to the interleukin-6 (IL-6) family. It is primarily secreted by activated T lymphocytes and macrophages as a 28 kDa glycoprotein. OSM shares properties with all members of this cytokine family, but is most closely related in structure and function to leukemia inhibitory factor (LIF). OSM acts on a variety of cells and is involved in regulating gene activation, cell growth, and inflammatory processes. It is a potent inducer of anti-proteases, has anti-inflammatory properties, acts as an immune modifier, and promotes the deposition of extracellular matrix proteins . In the context of respiratory diseases, OSM may play a significant role in normal wound healing, as well as in remodeling and pathological fibrosis.

Publications

Species	Application	Title
human	IF	Jpn J Ophthalmol; Role of oncostatin M in the pathogenesis of vernal keratoconjunctivitis: focus on tissue remodeling.; Authors - Keitaro Mashimo
mouse	IHC	Int J Radiat Oncol Biol Phys; Oncostatin M/Oncostatin M Receptor Signal Induces Radiation-Induced Heart Fibrosis by Regulating SMAD4 in Fibroblast; Authors - Peng Xu