OSCAR Polyclonal antibody

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  WB analysis using 21996-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21996-1-AP (OSCAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

OSCAR Polyclonal Antibody for WB, ELISA

Cat No. 21996-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Poly-Ig receptor 3, PIgR-3, PIgR3, Osteoclast-associated receptor, Osteoclast-associated immunoglobulin-like receptor

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Tested Applications

• Positive WB detected in: HL-60 cells, THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21996-1-AP targets OSCAR in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OSCAR fusion protein Ag17366
• Full Name: osteoclast associated, immunoglobulin-like receptor
• Calculated Molecular Weight: 282 aa, 31 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC035023
• Gene Symbol: OSCAR
• Gene ID (NCBI): 126014
• RRID: AB_3669383
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IYS5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Osteoclast-associated receptor (OSCAR) is an FcRγ-associated receptor that belongs to the leukocyte receptor cluster (LRC). OSCAR is expressed by myeloid cells and is involved in antigen presentation and activation of human dendritic cells(PMID: 15155468; PMID: 15650060). OSCAR is expressed in osteoclast cells and involved in the differentiation of osteoclasts from peripheral blood mononuclear cells (PBMC). The N-glycosylated form of human OSCAR has a molecular weight of approximately 40-45 kDa, while 30 kDa corresponds to its fully non-glycosylated form (PMID: 15155468; 24902903; 27917924)