OS9 Recombinant antibody

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  WB analysis using 82932-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82932-1-RR (OS9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 82932-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82932-1-RR (OS9 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 82932-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82932-1-RR (OS9 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using 82932-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug OS9 Recombinant antibody (82932-1-RR, Clone:230149B4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82932-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82932-1-RR against Human OS9 were performed. The affinity constant is below 1 pM.

OS9 Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 82932-1-RR

Clone No.230149B4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

Protein OS-9, Amplified in osteosarcoma 9, 230149B4

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Tested Applications

• Positive WB detected in: MCF-7 cells, HUVEC cells, U2OS cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82932-1-RR targets OS9 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: OS9 fusion protein Ag0106
• Full Name: osteosarcoma amplified 9, endoplasmic reticulum associated protein
• Calculated Molecular Weight: 76 kDa
• Observed Molecular Weight: 83-97 kDa
• GenBank Accession Number: BC000532
• Gene Symbol: OS9
• Gene ID (NCBI): 10956
• RRID: AB_3670673
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13438
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OS-9 is a ubiquitously expressed ensoplasmic reticulum (ER)-associated protein originally identified as being amplified in certain osteosarcomas. It functions in ER quality control and ER-associated degradation (ERAD). There are three isoforms of this protein: OS-9.1, OS-9.2 and OS-9.3. The longest isoform, OS-9.1, contains 667 aa, OS-9.2 lacks aa 535-589, whereas OS-9.3 lacks aa 456-470 and 535-589. OS-9.1 and OS-9.2 are N-glycosylated, ubiquitously expressed in human tissues, and amplified in tumors.