ORAI1 Polyclonal antibody
ORAI1 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 28637-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, A375 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:3000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
28637-1-AP targets ORAI1 in WB, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ORAI1 fusion protein Ag29779 |
Full Name | ORAI calcium release-activated calcium modulator 1 |
Calculated Molecular Weight | 301 aa, 33 kDa |
Observed Molecular Weight | 32 kDa, 45-50 kDa |
GenBank Accession Number | BC015369 |
Gene Symbol | ORAI1 |
Gene ID (NCBI) | 84876 |
RRID | AB_2881185 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Store-operated calcium channels (SOCs) play essential functions in various physiological processes. ORAI1 is a complex glycosylated protein, it exists in several molecular weight forms: a theoretical molecular weight of 32.7 kDa of the unmodified protein and two major glycosylated proteins of ~43 kDa and ~50 kDa (PMID: 22318387). Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.
Protocols
Product Specific Protocols | |
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WB protocol for ORAI1 antibody 28637-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |