Biotin-conjugated OPTN Monoclonal antibody

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• KD/KO Validated

OPTN Monoclonal Antibody for WB, IHC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC

Conjugate

Biotin

CloneNo.

6C1H4

Cat no : Biotin-60293

Synonyms

Optineurin, FIP2, FIP 2, FAA4, E3-14.7K-interacting protein

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Validation Data Gallery

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  WB analysis of human brain using Biotin-60293

  Fetal human brain was subjected to SDS-PAGE followed by western blot with Biotin-60293 (biotin labeled OPTN monoclonal antibody) at dilution of 1:1000. Avidin-HRP was used instead of secondary antibody.

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  IHC staining of human pancreas cancer using Biotin-60293

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using Biotin-60293 (OPTN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using Biotin-60293

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using Biotin-60293 (OPTN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human brain
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-60293 targets OPTN in WB, IHC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: OPTN fusion protein Ag1272
• Full Name: optineurin
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 70-74 kDa
• GenBank Accession Number: BC013876
• Gene Symbol: OPTN
• Gene ID (NCBI): 10133
• RRID: AB_2883065
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

OPTN, also named as FIP2, GLC1E, HIP7, HYPL and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN are the cause of primary open angle glaucoma type 1E (GLC1E). Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork. This antibody is conjugated with Biotin.