OPTN Recombinant antibody, PBS Only (Capture)

OPTN Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240636E8

Cat no : 83634-2-PBS

Synonyms

Optineurin, FIP2, FIP 2, FAA4, E3-14.7K-interacting protein

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00621-2

  Sandwich ELISA standard curve of MP00621-2, Human OPTN Recombinant Matched Antibody Pair - PBS only. 83634-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1272. 83634-3-PBS was HRP conjugated as the detection antibody. Range: 93.8-6000 pg/mL

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  Sample test of MP00621-2

  Jurkat and MCF-7 cell lysates were measured. The human OPTN concentration of detected samples was determined to be 29,772.0 pg/mL (based on a 1.0 mg/mL extract load) in Jurkat cell lysate and 8,643.8 pg/mL (based on a 4.3 mg/mL extract load) in MCF-7 cell lysate.

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  Sample test of MP00621-2

  Serum of sixteen individual healthy human donors was measured. The human OPTN concentration of detected samples was determined to be 813.0 pg/mL with a range of 92.0 - 5,037.9 pg/mL.

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  Cytometric bead array standard curve of MP00621-2

  Cytometric bead array standard curve of MP00621-2, OPTN Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83634-2-PBS. Detection antibody: 83634-3-PBS. Standard: Ag1272. Range: 1.25-80 ng/mL.

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  Cytometric bead array standard curve of MP00621-3

  Cytometric bead array standard curve of MP00621-3, OPTN Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83634-2-PBS. Detection antibody: 83634-1-PBS. Standard: Ag1272. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 83634-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 83634-2-PBS against Human OPTN was performed. The affinity constant is below 1 pM.

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Product Information

83634-2-PBS targets OPTN as part of a matched antibody pair:

MP00621-2: 83634-2-PBS capture and 83634-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00621-3: 83634-2-PBS capture and 83634-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: OPTN fusion protein Ag1272
• Full Name: optineurin
• Calculated Molecular Weight: 66 kDa
• GenBank Accession Number: BC013876
• Gene Symbol: OPTN
• Gene ID (NCBI): 10133
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.