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OPA3 Monoclonal antibody, PBS Only

OPA3 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5A3F9

Cat no : 68241-1-PBS

Synonyms

FLJ22187, FLJ25932, MGA3, OPA3, Optic atrophy 3 protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OPA3 Monoclonal antibody (68241-1-Ig)

    WB analysis using 68241-1-Ig (same clone as 68241-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68241-1-Ig (OPA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68241-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using OPA3 Monoclonal antibody (68241-1-Ig)

    WB analysis using 68241-1-Ig (same clone as 68241-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68241-1-Ig (OPA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68241-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68241-1-PBS targets OPA3 in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen OPA3 fusion protein Ag8173
Full Name optic atrophy 3 (autosomal recessive, with chorea and spastic paraplegia)
Calculated Molecular Weight 179 aa, 20 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC005059
Gene Symbol OPA3
Gene ID (NCBI) 80207
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The OPA3 cDNA encodes a deduced 179-amino acid protein. Northern blot analysis demonstrated a primary transcript of approximately 5.0 kb that was ubiquitously expressed, most prominently in skeletal muscle and kidney. Mutations in this gene have been shown to result in 3-methylglutaconic aciduria type III and autosomal dominant optic atrophy and cataract.